Kansas State University researchers are hoping to improve food safety by developing earlier and more accurate detection methods for the major genes specific for E. coli O157.
Lance Noll, master's student in veterinary biomedical science, T.G. Nagaraja, university distinguished professor of diagnostic medicine and pathobiology; and Jianfa Bai, assistant professor in the Kansas State Veterinary Diagnostic Laboratory, are leading the project to improve techniques for detecting pathogenic Shiga toxin-producing E. coli O157:H7.
A U.S. Department of Agriculture Coordinated Agriculture Project grant is funding the work.
The researchers are part of a College of Veterinary Medicine team studying preharvest food safety in beef cattle.
"Developing a method to detect E. coli before it can potentially contaminate the food supply benefits the beef industry by preventing costly recalls but also benefits the consumer by ensuring the safety of the beef supply," Noll said.
The newly developed test is a molecular assay, or polymerase chain reaction, that detects bacteria based on genetic sequences, which are the bacteria's "fingerprints," Nagaraja said. The test is rapid and less labor-intensive than existing detection methods. The method can be automated and test many samples in a short period of time.
The test can be used in a diagnostic or research laboratory to accurately detect E. coli and can help with quality control in cattle facilities.
"The novelty of this test is that it targets four genes," Nagaraja said. "We are constantly working on finding better and more sensitive ways to detect these pathogens of E. coli in cattle feces."
To develop the diagnostic test, Noll and Nagaraja worked with two Kansas State University molecular biologists: Xiaorong Shi, research assistant of diagnostic medicine and pathobiology, and Bai.
Noll was named a winner at the 11th annual Capitol Graduate Research Summit this spring for his research project and poster, "A four-plex real-time PCR assay for the detection and quantification of Escherichia coli O157 in cattle feces."